N-ethylmaleimide induces K+−H+antiport activity inEscherichia coliK-12
نویسندگان
چکیده
منابع مشابه
Inhibition of polyoma virus middle T antigen-associated tyrosyl kinase activity by N-ethylmaleimide.
The middle T antigen (MT Ag) encoded by polyoma virus has an associated protein kinase activity which transfers a phosphoryl group from ATP or GTP to a tyrosine residue on MT Ag in immunoprecipitates formed between polyoma virus-infected or transformed cell extracts and serum from animals bearing polyoma-induced tumors. Incubation of such immunoprecipitates or polyoma-transformed cell extracts ...
متن کاملSoluble N-ethylmaleimide-sensitive-factor attachment protein and N-ethylmaleimide-insensitive factors are required for Ca2+-stimulated exocytosis of insulin.
Ca2+ stimulates exocytosis in permeabilized insulin-secreting cells. To investigate the putative cytosolic components involved in the Ca2+ response, HIT-T15 cells (a pancreatic B-cell line) were permeabilized with streptolysin-O, a procedure that allows rapid exchange of soluble components including macromolecules. We found that in this cell preparation the secretory response to Ca2+ but not to...
متن کاملPharmacologic receptor activity of rabbit aorta. Effect of dithiothreitol and N-ethylmaleimide.
IV-ethylmaleimide (NEM), an alkylating agent that covalently binds sulfhydryl groups, and dithiothreitol (DTT, Cleland's reagent), which reduces disulfide bridges to sulfhydryl groups, affected drug-induced contractions of rabbit aorta. NEM depressed aortic responses to potassium chloride (KC1), norepinephrine, serotonin, histamine, and angiotensin II. This action was attributed to an interacti...
متن کاملNAPA ( N - ethylmaleimide - sensitive factor attachment protein , alpha )
Review on NAPA, with data on DNA/RNA, on the protein encoded and where the gene is implicated.
متن کاملPurification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport.
N-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. After inactivation of the Golgi membranes by NEM, transport can be rescued by adding back an appropriately prepared cytosol fraction. This complementation assay has allowed us to purify the NEM-sensitive factor, which we term NSF. The NEM-sensitive factor is a tetramer of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1982
ISSN: 0014-5793
DOI: 10.1016/0014-5793(82)80888-0